Covid-19 Multiplex RT-qPCR detection kit, developed at Inovia Technology laboratories, has specifically designed oligonucleotides that target gene regions that are not impacted by mutation, allowing it to detect SCoV-2 variants that have surfaced thus far, including the Omicron variant.
The probes of SARS-CoV-2 RDRP and E genes in the kit are labeled with FAM and CY5 fluorophores, respectively, while the probe of RNase P gene is labeled with HEX fluorophore as an internal control. The kit performs virus screening and extraction control in the same well in a multiplexed manner.
It is possible to control the quality of the oligonucleotides and detect possible contamination during the analysis using the reaction mix, primer-probe mix, positive control and negative control tubes provided in the kit.
Quantity in one package: 500.
The kit’s optimizations have been performed in the Inovialab GENX-4, Biorad CFX96, and Roche LC480, and results can be acquired in 47 minutes.
The Kit comes in two different package sizes: 100 and 500 reactions. Table 1 lists the components of both packages.
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